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CT-039

CatPure™ Cas9 mRNA

CatPure™ Cas9 mRNA

CatPure™ Cas9 mRNA (N1-Me-Pseudo UTP)
Size
Regular price $299.00 USD
Regular price Sale price $299.00 USD
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Length

4451 nt

Concentration

2 mg/mL

Buffer

MilliQ water

Storage Conditions

-65 C to -80 C

Documents

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Product description

Wild type Cas9 mRNA (N1-Me-Pseudo UTP) allows for the expression of a Streptococcus pyogenes SF370 Cas9 protein (CRISPR-associated protein 9), which is part of the CRISPR genome editing system, when transfected into cells. In the CRISPR system, when co-transfected with guide RNA (gRNA) targeting specific genomic loci, the Cas9 protein can cleave the targeted genes under the guidance of the gRNA. This product is an in vitro transcribed Cas9 mRNA that can be transfected at any time and used for CRISPR/Cas9-mediated gene editing.

High Integrity

mRNA integrity/purity analysis by capillary electrophoresis (CE)

High capping efficiency

mRNA 5’ capping efficiency analysis by LC/MS

mRNA Cell-free Translation

mRNA cell-free translation results by SDS-page

editing efficiency in 293T cell

T7 Endonuclease I digestion in 293T cell to verify Cas9 mRNA editing efficiency after cell transfection

Editing efficiency in mice

In vivo editing efficiency in mouse liver after IV injection of Cas9 mRNA lipid nanoparticles (LNP).