Wild type Cas9 mRNA (N1-Me-Pseudo UTP) allows for the expression of a Streptococcus pyogenes SF370
Cas9 protein (CRISPR-associated protein 9), which is part of the CRISPR genome editing system,
when transfected into cells. In the CRISPR system, when co-transfected with guide RNA (gRNA)
targeting specific genomic loci, the Cas9 protein can cleave the targeted genes under the guidance of the gRNA. This product is an in vitro transcribed Cas9 mRNA that can be transfected at any time and used for CRISPR/Cas9-mediated gene editing.
mRNA integrity/purity analysis by capillary electrophoresis (CE)
High capping efficiency
mRNA 5’ capping efficiency analysis by LC/MS
mRNA Cell-free Translation
mRNA cell-free translation results by SDS-page
editing efficiency in 293T cell
T7 Endonuclease I digestion in 293T cell to verify Cas9 mRNA editing efficiency after cell transfection
Editing efficiency in mice
In vivo editing efficiency in mouse liver after IV injection of Cas9 mRNA lipid nanoparticles (LNP).
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