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CT-072

CatPure™ FLuc mRNA

CatPure™ FLuc mRNA

CatPure™ Firefly Luciferase mRNA (N1-Me-Pseudo UTP)

Size
Regular price $299.00 USD
Regular price Sale price $299.00 USD
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Length

1913 nt

Concentration

1 mg/mL

Buffer

MilliQ water

Storage Conditions

-65 C to -80 C

Documents

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Product description

FLuc mRNA (N1-Me-Pseudo UTP) is a firefly luciferase encoded mRNA sequence, which can express luciferase protein in cells after transfection. The protein was originally extracted from the firefly (Photinus pyralis). Luciferase can catalyze the oxidation of luciferin to produce oxyluciferin, emitting yellow-green bioluminescence (wavelength of 550-570 nm), and the light intensity directly reflects the expression level of luciferase. This product can serve as a positive control for mRNA transfection, and further be used for screening and validation of delivery formulations or expression systems.

High Integrity

mRNA integrity/purity analysis by capillary electrophoresis (CE)

High capping efficiency

mRNA 5’ capping efficiency analysis by LC/MS

mRNA Cell-free Translation

mRNA cell-free translation results by SDS-page

mRNA expression in HeLa Cell

RFU quantitative results of firefly luciferase expression in HeLa cell at 48h after cell transfection

mRNA expression in mice

 CatPure™ FLuc mRNA translation in mice via in vivo protein express assay in comparison with market competitors.

mRNA expression in mice

CatPure™ FLuc mRNA potency in mice measured by in vivo imaging at 6h, 12h, and 48h after injection of CatPure™ FLuc mRNA.