GLuc mRNA (N1-Me-Pseudo UTP) is a Gaussia luciferase-encoded mRNA sequence, which can
express the bioluminescent protein in cells after transfection. The protein was originally extracted from a marine copepod (Gaussia princeps). Gaussia luciferase can catalyze the oxidation of substrate coelenterazine, resulting in light emission at a wavelength of 480nm,
and the light intensity is directly proportional to the expression level of GLuc. This product can serve as a positive control for mRNA transfection, and further be used for screening and validation of delivery formulations or expression systems.
mRNA integrity/purity analysis by capillary electrophoresis (CE)
High capping efficiency
mRNA 5’ capping efficiency analysis by LC/MS
mRNA Cell-free Translation
mRNA cell-free translation results by SDS-page
mRNA expression in HeLa Cell
RFU quantitative results of Gaussia Luciferase expression in HeLa cell at 48h after cell transfection
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