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CT-084

CatPure™ GLuc mRNA

CatPure™ GLuc mRNA

CatPure™ Gaussia luciferase mRNA (N1-Me-Pseudo UTP)

Size
Regular price $299.00 USD
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Length

818 nt

Concentration

2 mg/mL

Buffer

MilliQ water

Storage Conditions

-65 C to -80 C

Documents

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Product description

GLuc mRNA (N1-Me-Pseudo UTP) is a Gaussia luciferase-encoded mRNA sequence, which can express the bioluminescent protein in cells after transfection. The protein was originally extracted from a marine copepod (Gaussia princeps). Gaussia luciferase can catalyze the oxidation of substrate coelenterazine, resulting in light emission at a wavelength of 480nm, and the light intensity is directly proportional to the expression level of GLuc. This product can serve as a positive control for mRNA transfection, and further be used for screening and validation of delivery formulations or expression systems.

High Integrity

mRNA integrity/purity analysis by capillary electrophoresis (CE)

High capping efficiency

mRNA 5’ capping efficiency analysis by LC/MS

mRNA Cell-free Translation

mRNA cell-free translation results by SDS-page

mRNA expression in HeLa Cell

RFU quantitative results of Gaussia Luciferase expression in HeLa cell at 48h after cell transfection