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AA-MRNA002

EGFP mRNA

EGFP mRNA

Enhanced Green Fluorescent Protein mRNA (N1-Me-Pseudo UTP).

Size
Regular price $299.00 USD
Regular price Sale price $299.00 USD
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Quantity

Length

980 nt

Concentration

1 mg/mL

Buffer

MilliQ water or 1mM sodium citrate pH 6.5

Storage Conditions

-20°C for short term (<3 months) storage and -80°C for long term storage.

Documents

Pardon the dust, work in progress.

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Product description

EGFP mRNA (N1-Me-Pseudo UTP) is capable of expressing enhanced green fluorescent protein (EGFP) within cells following transfection. EGFP was originally extracted from the jellyfish (Aequorea Victoria), and is a commonly used reporter gene for direct detection in mammalian cell culture. It produces bright green fluorescence (at a wavelength of 509 nm). This product can serve as a positive control for mRNA transfection, and further be used for screening and validation of delivery formulations or expression systems.

This mRNA features following structure: 

  • Cap 1 (N7-methylguanosine Cap) with high capping efficiency 
  • Poly(A) tail with 100 consecutive A 
  • 5’ and 3’ UTR with proven efficient translation 
  • ORF with codon optimization
  • N1-methyl-pseudouridine modification

Competitive advantage of AmRNA product:

At AmRNA, we are committed to delivering ready-to-use mRNA of the highest quality standards (only A grade). Each mRNA is produced using state-of-the-art industrial synthesis and purification technologies, ensuring ultra-high purity, capping efficiency, and poly(A) tail integrity. Residual impurities such as protein, DNA, and dsRNA are reduced to trace levels to minimize potential immunogenicity. Our comprehensive quality control panel rigorously verifies every critical attribute to ensure batch-to-batch consistency, ensuring data reproducibility in both in vitro and in vivo applications.

Quality Control Specifications


Quality Attributes QC Method Release Standard
Appearance Visual inspection Clear and free of foreign particles
pH pH meter Target +/- 0.2 
Content UV-Vis Target +/- 5%
A260/280 UV-Vis 1.8-2.2
Identity Sequencing Sequence fully match
Integrity/Purity

Capillary electrophoresis 

>90.0%
Capping Efficiency LC-MS >95.0%
Tailed% HPLC >95.0%
dsRNA ELISA <0.1%
Endotoxin

LAL assay 

<10 EU/mg

Applications

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Need a different NTP modification, sequence insertion or codon optimization?